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access array system technology  (fluidigm)


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    Structured Review

    fluidigm access array system technology
    Access Array System Technology, supplied by fluidigm, used in various techniques. Bioz Stars score: 93/100, based on 843 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/access+array+technology/Access+Array/pm41735033-79-8-12
    Average 93 stars, based on 843 article reviews
    access array system technology - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    DNA Sequencing:

    Article Title: Decrease in Effective Population Size After the Immigration of Asian Black Bears to Japan
    Article Snippet: .. Genomic DNA was extracted from each tissue sample using the MagExtractor (TOYOBO), and double digest restriction- site associated DNA sequencing (RAD- seq) was conducted following a protocol that combines the standard method of Peterson et al. (2012) with the amplicon tagging protocol for Access Array technology (Fluidigm, South San Francisco, CA, USA). ..

    Amplification:

    Article Title: Decrease in Effective Population Size After the Immigration of Asian Black Bears to Japan
    Article Snippet: .. Genomic DNA was extracted from each tissue sample using the MagExtractor (TOYOBO), and double digest restriction- site associated DNA sequencing (RAD- seq) was conducted following a protocol that combines the standard method of Peterson et al. (2012) with the amplicon tagging protocol for Access Array technology (Fluidigm, South San Francisco, CA, USA). ..

    Next-Generation Sequencing:

    Article Title: Leukocytosis, monocytosis, and eosinophilia in systemic mastocytosis: analysis of phenotype, genetics and prognosis in 596 patients from the GREM registry.
    Article Snippet: 107 108 Mutational analyses 109 For quantitative assessment of the KIT D816V EAB, allele-specific quantitative real-time PCR analyses 110 were performed as previously described. (20) Next-generation deep amplicon sequencing by 454 FLX 111 amplicon chemistry (Roche, Penzberg, Germany) was performed to investigate 18 candidate genes 112 known as hotspot regions in myeloid neoplasms as previously described. .. The sequential NGS approach 113 was based on library preparation by the Access Array Technology (Fluidigm, San Francisco, CA) and 114 sequencing on the MiSeq Instrument (Illumina, San Diego, CA). ..

    Article Title: The new provisional WHO entity ‘ RUNX1 mutated AML' shows specific genetics but no prognostic influence of dysplasia
    Article Snippet: 140/152 samples were investigated by a next generation sequencing (NGS) approach based on library preparation by the Access Array technology (Fluidigm, San Francisco, CA) and sequencing on the MiSeq Instrument (Illumina, San Diego, CA). .. 140/152 samples were investigated by a next generation sequencing (NGS) approach based on library preparation by the Access Array technology (Fluidigm, San Francisco, CA) and sequencing on the MiSeq Instrument (Illumina, San Diego, CA). ..

    Article Title: Response and resistance to cladribine in patients with advanced systemic mastocytosis: a registry-based analysis.
    Article Snippet: .. NGS analyses on DNA were performed through library preparation by the Access Array Technology (Fluidigm, San Francisco, CA) and sequencing on the MiSeq Instrument (Illumina, San Diego, CA). .. Gene mutations were annotated using the reference sequence of the Ensembl Transcript ID (Ensembl release 85: July 2016).

    Article Title: Clinical, morphological and genetic characteristics of patients with concurrent presence of JAK2 V617F and BCR::ABL1
    Article Snippet: The volume included 7.1 μL of 10 ng/μL DNA, 7.5 μL of QuantStudioTM 3D Digital PCR Master Mix v2 (ThermoFisher Scientific, Waltham, MA, USA) and 0.4 μL of JAK2 V617F specific TaqMan gene expression assay (ID: Hs000000038_rm, ThermoFisher ScientificWaltham, MA, USA). dPCR was performed using the following thermal cycling conditions: 96 °C for 10 min, (56 °C for 2 min, 98 °C for 30 s (x39 cycles)) and 56 °C for 2 min. .. Next generation sequencing (NGS) of a myeloid gene panel of 18 frequently mutated genes in MPN was performed through library preparation by the Access Array Technology (Fluidigm, San Francisco, CA) and sequencing on the MiSeq Instrument (Illumina, San Diego, CA). ..

    Sequencing:

    Article Title: Leukocytosis, monocytosis, and eosinophilia in systemic mastocytosis: analysis of phenotype, genetics and prognosis in 596 patients from the GREM registry.
    Article Snippet: 107 108 Mutational analyses 109 For quantitative assessment of the KIT D816V EAB, allele-specific quantitative real-time PCR analyses 110 were performed as previously described. (20) Next-generation deep amplicon sequencing by 454 FLX 111 amplicon chemistry (Roche, Penzberg, Germany) was performed to investigate 18 candidate genes 112 known as hotspot regions in myeloid neoplasms as previously described. .. The sequential NGS approach 113 was based on library preparation by the Access Array Technology (Fluidigm, San Francisco, CA) and 114 sequencing on the MiSeq Instrument (Illumina, San Diego, CA). ..

    Article Title: The new provisional WHO entity ‘ RUNX1 mutated AML' shows specific genetics but no prognostic influence of dysplasia
    Article Snippet: 140/152 samples were investigated by a next generation sequencing (NGS) approach based on library preparation by the Access Array technology (Fluidigm, San Francisco, CA) and sequencing on the MiSeq Instrument (Illumina, San Diego, CA). .. 140/152 samples were investigated by a next generation sequencing (NGS) approach based on library preparation by the Access Array technology (Fluidigm, San Francisco, CA) and sequencing on the MiSeq Instrument (Illumina, San Diego, CA). ..

    Article Title: Frequency of Pathogenic Germline Variants in <i>CDH1, BRCA2, CHEK2, PALB2, BRCA1</i>, and <i>TP53</i> in Sporadic Lobular Breast Cancer
    Article Snippet: .. Methods: Access Array technology (Fluidigm) was used to amplify all exons of CDH1, BRCA2, BRCA1, TP53, CHEK2, and PALB2 using a custommade targeted sequencing panel in 1,434 cases of ILC and 368 cases of pure LCIS together with 1,611 controls. ..

    Article Title: Frequency of Pathogenic Germline Variants in <i>CDH1, BRCA2, CHEK2, PALB2, BRCA1</i>, and <i>TP53</i> in Sporadic Lobular Breast Cancer
    Article Snippet: .. After DNA extraction from peripheral blood, Access Array technology (Fluidigm) was used to amplify all exons of CDH1, BRCA2, BRCA1, TP53, CHEK2, and PALB2 using a custom-made targeted sequencing panel consisting of 321 amplicons (Supplementary Table S1). .. Purified libraries were quantified using Qubit High Sensitivity Assay Kit and sequenced on a HiSeq2500 (Illumina).

    Article Title: Response and resistance to cladribine in patients with advanced systemic mastocytosis: a registry-based analysis.
    Article Snippet: .. NGS analyses on DNA were performed through library preparation by the Access Array Technology (Fluidigm, San Francisco, CA) and sequencing on the MiSeq Instrument (Illumina, San Diego, CA). .. Gene mutations were annotated using the reference sequence of the Ensembl Transcript ID (Ensembl release 85: July 2016).

    Article Title: Clinical, morphological and genetic characteristics of patients with concurrent presence of JAK2 V617F and BCR::ABL1
    Article Snippet: The volume included 7.1 μL of 10 ng/μL DNA, 7.5 μL of QuantStudioTM 3D Digital PCR Master Mix v2 (ThermoFisher Scientific, Waltham, MA, USA) and 0.4 μL of JAK2 V617F specific TaqMan gene expression assay (ID: Hs000000038_rm, ThermoFisher ScientificWaltham, MA, USA). dPCR was performed using the following thermal cycling conditions: 96 °C for 10 min, (56 °C for 2 min, 98 °C for 30 s (x39 cycles)) and 56 °C for 2 min. .. Next generation sequencing (NGS) of a myeloid gene panel of 18 frequently mutated genes in MPN was performed through library preparation by the Access Array Technology (Fluidigm, San Francisco, CA) and sequencing on the MiSeq Instrument (Illumina, San Diego, CA). ..

    other:

    Article Title: The nucleoside-diphosphate kinase NME3 associates with nephronophthisis proteins and is required for ciliary function during renal development
    Article Snippet: Targeted amplification was performed by multiplexed PCR using Fluidigm Access-ArrayTM technology followed by barcoding and next-generation resequencing on an IlluminaTM MiSeq platform, as previously established by our group (60).

    DNA Extraction:

    Article Title: Frequency of Pathogenic Germline Variants in <i>CDH1, BRCA2, CHEK2, PALB2, BRCA1</i>, and <i>TP53</i> in Sporadic Lobular Breast Cancer
    Article Snippet: .. After DNA extraction from peripheral blood, Access Array technology (Fluidigm) was used to amplify all exons of CDH1, BRCA2, BRCA1, TP53, CHEK2, and PALB2 using a custom-made targeted sequencing panel consisting of 321 amplicons (Supplementary Table S1). .. Purified libraries were quantified using Qubit High Sensitivity Assay Kit and sequenced on a HiSeq2500 (Illumina).



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